II Brazil-Argentine Microfluidics Congress
V Congreso de Microfluídica Argentina
October 22-25, 2019. Córdoba, Argentina.
School of Chemical Engineering, University of Campinas
UNICAMP Microfluidics Laboratory Head
Professor at Department of Materials and Bioprocess Engineering
Campinas, São Paulo, Brazil
Professor Lucimara Gaziola de la Torre is graduated in Chemical Engineering (1994) and has a Ph.D. in Chemical Engineering (2006) by the University of Campinas. She is Professor in the Department of Materials and Bioprocess Engineering, School of Chemical Engineering, UNICAMP (November 2009). She completed research missions at the Victoria University/Canada (2019), École Polythechnique (Paris) (2015), the National Institute of Standards, Technology (NIST) in Gaithersburg, USA (July 2012) and the Institute of Technical Biochemistry (ITB) (1995). She has experience in the industry, working in the food sector, in chemical specialties and in biotechnological processes (1995-2001). Her research focuses on the development of biotechnological processes, especially in the field of nano & biotechnology. Microfluidics is explored as a potential technology to investigate the mechanisms of nanoparticles formation, synthesis of hybrid microparticles and encapsulation of bioactive molecules. Microdevices are also explored to observe microbial and mammalian cells behavior aiming at the determination of kinetic growth parameters, development of systems for drug screening and new strategies for biological in vitro models.
Talk title: "Microfluidics applied to Nano, Micro and Biotechnology"
Microfluidics is a multidisciplinary field that operates at the microscale with small amount of fluids. The hydrodynamic characteristics allow the control of chemicals, cells, lipids, and nucleic acids in space and time. Therefore, microfluidics enables applications that would not be feasible at the macroscale and this platform demonstrates high potentiality as a tool for in vitro cell culture, biological models, drug research, synthesis of nanomaterials and microencapsulation. The microenvironment uses few amounts of sample and reagents that flows in microchannels in the laminar regime since no external force is applied. In this case, the micron scale minimizes effects of mass and heat transfer, allowing integration of different techniques for data acquisition in different ways than macro scale. These characteristics open great opportunities for technological research in the fields of Micro/Nano & Biotechnology. This presentation will explore different applications of microfluidics in the field of Micro/Nano & Biotechnology.
Laboratorio de Medios e Interfases, Dpto. De Bioingeniería, FACET, University of Tucumán
Instituto Superior de Investigaciones Biológicas (INSIBIO)- CONICET
Rossana Madrid is EE and Ph.D. in Bioengineering. She is currently Full Professor of Biomedical Transducers and Biosensors and Microsystems of the Biomedical Engineering Program, and also at the Doctoral degree, at the Faculty of Science and Technology at the University of Tucumán, Argentina. She is Independent Researcher at the National Council of Scientific and Technical Investigations of Argentina (CONICET). She has published R&D papers in national and international journals, two chapters in books and has developed two patents. She leads research grants and international cooperation grants with two German Universities. She leads graduate and postgraduate theses in the area of biosensors and microfluidics. Her main research fields include sensors and biosensors, microfluidic systems and paper-based POC (Point of care) devices for biomedical and environmental applications. Particularly, her microfluidic group is working now in the development, from one side, of a microfluidic paper POC device for the detection of Leishmaniasis in cooperation with the Experimental Pathology Institute of Salta, and on the other hand, of hybrid microfluidic chips. For the latter case, the incorporation of active elements (peristaltic pumps, valves, etc.) made with stimulus-sensitive hydrogels for fluid management, is evaluated. In the area of biosensors, her group won in February 2019, the Eukera prize to make the field validation of a biosensor for the early detection of HuangLongBing disease (HLB) in citrus plants.
Talk title: "Biosensors in Microfluidic Devices: Recent advances and future trends"
Claudia Trejo is a bachelor in physical sciences from the Universidad de Concepción and master in Physical Science from the Instituto Balseiro. She obtained her Ph.D. in Physics at the Universitat de Barcelona in 2016 where she worked with the group of Dynamics of interfaces in nanotechnology, fluidics and biophysics. She has published R&D papers in international journals, colaborate in technological transfer projects with the Laboratory of Complex Fluids of the Centre de Recerca Matemàtica, and develops as a consultant for RheoDX inc.
Since 2018 she is developing her research at the Instituto de Física of the Pontificia Universidad Católica de Valparaíso where she is in charge of an international collaboration project with Barcelona and leads the Biorheology and Microfluidics Laboratory. Here with her group they develop experimental research in interface dynamics in microfluidics, hemorheology, red blood cells dynamics in confined geometries and mechanical properties of red blood cells. Currently, in collaboration with the Institute of Chemistry and the Medical Technology departament of PUCV, she is working on the development of Point of Care diagnostics techniques, relating the viscosity of blood with the mechanical properties of red blood cells.
Talk title: "Viscosity of blood and its relation with the mechanical properties of red blood cells"
Professor Fossum graduated with MSc in physics from NTH (presently NTNU) in 1978, and PhD in physics from NTH in 1983, followed by postdocoral appointments at Universite de Sherbrooke, Quebec, Canada (1984-85) and MIT- Massachusetts Institute of Technology, Chemistry and Physics Departments, USA (1985- 89). Since 2003 Fossum has been Full Professor at the Department of Physics, NTNU, including a number of sabattical stays: ESPCI-ParisTech, France partly invited to the Joliot Chair (2004 and 2016), UFPE-Recife, Brazil (2008), Centre for Advanced Studies (CAS) at the Norwegian Academy of Science and Letters (2011-12), and at PUC-Rio de Janeiro (2012-15) as part time visiting scientist under a Conselho Nacional de Desenvolvimento Científico e Tecnológico Ciência sem Fronteiras project.
Prof. Patrick Tabeling is leader of the group MMN (Microfluidics MEMS and Nanostructures), a prominent team in the field of microfluidics. He is the cofounder of the startup MicroFactory. He occupied various positions at the University: visiting researcher in Chicago University (1984-1985), Chargé/Directeur de Recherches CNRS in the Department of Physics in ENS (1985-2001), visiting professor to UCLA, Directeur de Recherches/Professor at ESPCI. He is cofounder of the Institut Pierre-Gilles de Gennes, and has been its director for the period July 2011-July 2018. He was professor chargé de cours at the Ecole Polytechnique (1996-2008). He is the author of 200 papers, 11 patents, 80 invited talks in international conferences; he was divisional editor of Physical Review Letters, Associate Editor to Physics of Fluids, PRF. He is member of Academia Europae. He published the book entitled “An introduction to microfluidics” (Oxford University Press – a French version being edited by Belin) in 2005.
Sorin Melinte is Professor of Electrical Engineering in the Institute of Information and Communication Technologies, Electronics and Applied Mathematics at Université catholique de Louvain, Belgium. His current projects aim at developing novel nano- and micro-devices and hybrid inorganic-organic platforms for molecular electronics as well as scaffolds for bioelectronics and additive manufacturing via microfluidics. In particular, he uses high-resolution electron-beam nanolithography and soft-lithography to link bottom-up and top-down nano- and micro-structuring techniques for the engineering of smart materials.
Talk title: "Novel electro-optic techniques and nano-engineered transparent materials for microfluidics"
Designed with targeted properties and combining multiple attributes that allow them to be dynamic, ordered particle assemblies are strong candidates to replace the traditional structures processed with top-down fabrication approaches. Self-assembly by gravitational sedimentation is one of the methods largely used to organize particles in desired architectures. Understanding the physical phenomena that take place during self-assembly is a crucial step in controlling the structure and properties of the colloid-designed materials. In this respect, novel approaches to analyse the spatial and temporal information embedded in colloidal systems confined to microfluidic environments are needed.
Interestingly, coplanar waveguides can be used to study the physical phenomena that appear during gravitational sedimentation due to their unique response towards the dielectric properties of the surrounding environment. Microwave measurements using coplanar waveguides were reported for fluidic environments, with a focus on the dielectric characteristics of liquid samples. Various other electro-optic techniques and devices were designed at microwave frequencies to measure dielectric properties of biomaterials or analyte concentrations in microfluidic systems: coaxial probes, microstrip transmission lines or microwave resonators. We employ coplanar waveguides and simple microfluidic structures to study the on-chip sedimentation of polystyrene nano- and micro-spheres in aqueous solutions. Along with the detection of the solution's concentration, we monitor the time evolution of the underlying sedimentation processes and we devise a technique applicable to the specific detection of molecular species. We present a novel class of biosensing devices, based on coplanar waveguides fabricated onto thin glass and working in the frequency interval from 40 MHz to 40 GHz.
Finally, we discuss further integration of coplanar waveguides within lab-on-chip technologies. We address nano-engineered transparent electronics and the exploitation of complex circuitry for enhanced real-time monitoring of target analytes as well as for surveying soft matter self-assembly in various microfluidic environments.